Coding

Part:BBa_K4131005:Design

Designed by: Chun Ying Yu   Group: iGEM22_CCU_Taiwan   (2022-10-09)


Original pfa C gene (Moritella marina)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3490
    Illegal XbaI site found at 4746
    Illegal PstI site found at 671
    Illegal PstI site found at 3427
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3490
    Illegal PstI site found at 671
    Illegal PstI site found at 3427
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3490
    Illegal BglII site found at 5238
    Illegal XhoI site found at 1366
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3490
    Illegal XbaI site found at 4746
    Illegal PstI site found at 671
    Illegal PstI site found at 3427
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3490
    Illegal XbaI site found at 4746
    Illegal PstI site found at 671
    Illegal PstI site found at 3427
    Illegal AgeI site found at 4246
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No modification is made in the sequence


Source

The sequence is extracted from the region 1623835-1629870 in Moritella marina genome (WP_019440098.1)

References